Extração de lipídios da microalga Scenedesmus sp. Com diferentes mistura de solventes orgânicos

  • JOSÉ JOVANNY BERMUDEZ SIERRA Universidade Federal de Ceara.
Palabras clave: Ácidos grasos, Rompimiento celular, Microalgas, Constante Dieléctrica, Derivatización.

Resumen

Las microalgas son fuentes de lípidos con potencial en producir biocombustibles y suplementos alimentarios en gran escala. El presente estudio tiene como objetivo evaluar el papel de los solventes orgánicos apolares y próticos polares (proporción 1:2) en la extracción y cuantificación de lípidos totales y ésteres metílicos de ácidos grasos (FAMEs sigla en inglés) en la microalga Scenedesmus. sp. A las muestras liofilizadas fueron cuantificados los lípidos por Blight & Dyer (B&D) modificado y pre-tratadas con ácido clorhídrico 3 mol.L-1 (con y sin HCl). El cloroformo fue reemplazado por acetato de etilo, éter de petróleo y éter etílico, y el metanol por etanol, isopropanol y terc-butanol. Los FAMEs en los tratamientos, con o sin adición de HCl, fueron determinados por cromatografía de gases. Los resultados evidenciaron que la mayor extracción de lípidos y FAMEs con HCl en los tratamientos se explica por rompimiento de la celular y liberación de los lípidos. Los solventes orgánicos y los alcoholes mostraron una eficiencia adecuada en presencia de HCl, igualmente el éter de petróleo y etanol demostraron resultados similares con B&D utilizado como referencia. En conclusión, la combinación entre los solventes apolares e próticos polares aumentaron la extracción y los rendimientos, especialmente cuando el éter de petróleo o el éter etílico con etanol (HCl), proporcionando los FAMEs insaturados de lípidos neutros.

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Biografía del autor/a

JOSÉ JOVANNY BERMUDEZ SIERRA, Universidade Federal de Ceara.

Estudante pós-graduação Doutorado em Biotecnologia
“RENORBIO”.

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Cómo citar
BERMUDEZ SIERRA, J. J. (2018). Extração de lipídios da microalga Scenedesmus sp. Com diferentes mistura de solventes orgânicos. Biotecnología En El Sector Agropecuario Y Agroindustrial, 16(2), 88–98. https://doi.org/10.18684/bsaa.v16n2.1169
Publicado
2018-07-01
Sección
Artículos de Investigaciòn
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