Bioprospeccion de bacterias productoras de polihidroxialcanoatos (pha's) en el departamento de Nariño

  • Ivan Dario Otero Universidad de Nariño.
  • Pablo Fernandez Universidad de Nariæo.
Palabras clave: Suelos de Nariño, Laguna de la Cocha, Ro Pasto, Polihidroxibutirato, Polihidroxivalerato

Resumen

Los polihidroxialcanoatos (PHA's), gránulos intracelulares acumulados por bacterias en ambientes con desbalance Carbono/Nitrógeno, Carbono/Fósforo tienen propiedades físico-químicas similares a plásticos convencionales, pero son biodegradables. Sin embargo, los elevados costos de producción de los PHA's conllevan a la búsqueda de estrategias con la menor inversión posible; por ello, en esta investigación se exploraron diferentes ambientes del departamento de Nariño en búsqueda de bacterias productoras de PHA's y se seleccionaron aquellas con potencial industrial. La detección presuntiva de bacterias PHA's a partir de muestras de suelo y agua, se realizó utilizando Rojo Nilo, los aislamientos seleccionados se sometieron a una fermentación Batch y el polímero obtenido se caracterizó por cromatografía de gases y cromatografía de gases-espectrometría de masas. Aquellos aislamientos con mayor acumulación de polímero se caracterizaron mediante pruebas bioquímicas y la secuencia parcial del gen rRNA 16S.Finalmente, se evaluó la producción de PHA's utilizando como sustrato aguas residuales domésticas. Se obtuvo 189 aislamientos bacterianos que produjeron desde 0,01gL-1 hasta 5,24gL-1 del homopolimero Poli-3-hidroxibutirato o el copolímero Poli-3-hidroxibutirato-co-3-hidroxivalerato. Así mismo, se recuperó 0,111gL-1 de Poli-3-hidroxibutirato a partir de las aguas residuales. Desde esta perspectiva las bacterias silvestres productoras de PHA's del departamento de Nariño tienen potencial en procesos ambientales e industriales.

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Biografía del autor/a

Ivan Dario Otero, Universidad de Nariño.
Biologo, Investigador Grupo de Biotecnologa Microbiana,
Pablo Fernandez, Universidad de Nariæo.
Ph.D. Ciencias Biolgicas `rea Microbiologa. Director Grupo de Biotecnologa Microbiana,

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Cómo citar
Otero, I. D., & Fernandez, P. (2013). Bioprospeccion de bacterias productoras de polihidroxialcanoatos (pha’s) en el departamento de Nariño. Biotecnología En El Sector Agropecuario Y Agroindustrial, 11(1), 12–20. Recuperado a partir de https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/1221
Publicado
2013-11-01
Sección
Artículos de Investigaciòn
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