Actividad antioxidante e influencia del periodo climático sobre el contenido de polifenoles totales en Merremia aegyptia
Palabras clave:
Bioprospección, Metabolitos secundarios, Convolvulaceae, La Guajira
Resumen
Merremia aegyptia (Convolvulaceae) no se ve afectada por la aridez que presenta la mayor parte del territorio guajiro (La Guajira, Colombia). Sin embargo, ha sido subestimada y considerada arvense al desconocerse sus propiedades medicinales y otros posibles usos. Así, con la presente investigación se pretendió establecer la influencia de los periodos climáticos y la fenología sobre la producción de polifenoles en dicha especie y estimar el potencial antioxidante de sus extractos. Se tomaron muestras de tejido foliar durante un año y se sometieron a extracción con metanol, la concentración de polifenoles totales fue establecida mediante el método de Folin-Ciacolteau y se correlacionó con la precipitación y temperatura mensual. Además, se estimó el porcentaje de inhibición de los extractos sobre el radical DPPH. Los resultados obtenidos indicaron variación en la concentración mensual de polifenoles totales, siendo enero de 2018 el mes donde se registró la mayor concentración (6,03%). Adicionalmente, los extractos presentaron mayor actividad antioxidante (85,15%) que el ácido ascórbico (38,65%). Se concluyó que la disponibilidad de agua en el suelo afecta la producción de polifenoles en M. aegyptia y que esta especie representa un recurso vegetal susceptible de ser cultivado y explotado bajo las condiciones ambientales de La Guajira.
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Disciplinas:
Biotecnología, bioprospecciónLenguajes:
BatatillaReferencias bibliográficas
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[27] ISLAM, M., YOSHIMOTO, M., YAHARA, S., OKUNO, S., ISHIGURO, K. and YAMAKAWA, O. Identification and characterization of foliar polyphenolic composition in sweetpotato (Ipomoea batatas L.) genotypes. Journal of Agricultural and Food Chemistry, 50(13), 2002, p. 3718–3722.
[2] JOSHI, R., AGARWAL, S. and PATNI, V. Evaluation of anti-microbial activity of in vitro and in vivo plant parts of Merremia Dissecta and Merremia Aegyptia. International Journal of Pharmaceutical Sciences and Research, 6(6), 2015, p. 2477-2483.
[3] OMOTAYO, F. and BOROKINI, T. Comparative phytochemical and ethnomedicinal survey of selected medicinal plants in Nigeria. Scientific Research and Essays, 7(9), 2012, p. 989-999.
[4] PACHECO, D., STHORMES, G., PETIT, Y., QUIRÓS, M., POLEO, N. and DORADO, I. Reconocimiento de malezas presentes en el huerto de guayabo (Psidium guajava L.) tipo Criolla Roja, del Centro Frutícola del Zulia, Municipio Mara, Venezuela. Revista UDO Agrícola, 9(1), 2009, p. 141-147.
[5] ISLAM, I., SHAIKH, A. and SHAHIDUL, I. Antioxidative and antimutagenic potentials of phytochemicals from Ipomoea batatas (L) Lam. Internacional Journal of Cancer Research, 5(3), 2009, p. 83-94.
[6] HOSSAIN, M.A. and SHAH, M.D. A study on the total phenols content and antioxidant activity of essential oil and different solvent extracts of endemic plant Merremia borneensis. Arabian Journal of Chemistry, 8, 2015, p. 66–71.
[7] KARNAN, R. and SUBRAMANI, K. Antioxidant properties of certain South Indian medicinal plants. Journal of Medicinal Plants Studies, 3(2), 2015, p. 58-60.
[8] KUMAR, P.R. Application of HPLC and ESI-MS techniques in the analysis of phenolic acids and flavonoids from green leafy vegetables (GLVs). Journal of Pharmaceutical Analysis, 7, 2017, p. 349–364.
[9] KAVERY, K. and JEYABALAN, G. Optimization of extraction method and profiling of plant phenolic compounds of Merremia umbellata (hallier. f.) through RP-HPLC. Global Journal for Research Analysis, 8(7), 2019, p. 146-147.
[10] KHAN, R., KHAN, M., SAHREEN, S. and AHMED, M. Evaluation of phenolic contents and antioxidant activity of various solvent extracts of Sonchus asper (L.) Hill. Chemistry Central Journal, 6, 2012, p. 12.
[11] NETO, F., DE OLIVEIRA, L., DOS SANTOS, A., DE LIMA, J. e SILVA, I. Otimização agroeconômica da cenoura fertilizada com diferentes doses de jitirana. Revista Ciência Agronômica, 45(2), 2014, p. 305-311.
[12] COLOMBIA. INSTITUTO DE HIDROLOGÍA, METEOROLOGÍA Y ESTUDIOS AMBIENTALES (IDEAM). Cartas climatológicas mensuales, Aeropuerto Almirante Padilla (Riohacha). Subdirección de meteorología [online]. 2018. Disponible: http://bart.ideam.gov.co/cliciu/rioha/tabla.htm. [citado 28 de enero de 2018].
[13] HUE, S., BOYCE, A. and CHANDRAN, S. Comparative study on the antioxidant activity of leaf extract and carotenoids extract from Ipomoea batatas var. Oren (Sweetpotato) leaves. World Academy of Science, Engineering and Technology, 58, 2011, p. 584-587.
[14] NAGAI, M., TANI, M., KISHIMOTO, Y., IIZUKA, M., SAITA, E., TOYOZAKI, M., KAMIYA, T., IKEGUCHI, M. and KONDO, K. Sweet potato (Ipomoea batatas L.) leaves suppressed oxidation of low density lipoprotein (LDL) in vitro and in human subjects. Journal of Clinical Biochemistry and Nutrition, 48(3), 2011, p. 203–208.
[15] AUSTRIA. IAEA (International Atomic Energy Agency). Quantification of tannins in tree foliage. FAO/IAEA working document. Viena (Austria): 2000, 26 p.
[16] INTERNATIONAL CONFERENCE ON HARMONISATION OF TECHNICAL REQUIREMENTS FOR REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE. ICH Harmonised Tripartite Guideline, validation of analytical procedures: text and methodology Q2(R1). Current Step 4 version, Parent Guideline dated 27 October 1994 (Complementary Guideline on Methodology dated 6 November 1996 incorporated in November 2005). 2005, 17 p.
[17] FARRUKH, A., IQBAL, A. and ZAFAR, M. Antioxidant and free radical scavenging properties of twelve traditionally used indian medicinal plants. Turkish Journal of Biology, 30, 2006, p. 177-183.
[18] POP, A., TOFANA, M., SOCACI, S., VÂRBAN, D., NAGY, M., BORŞ, M. and SFECHIŞ, S. Evaluation of antioxidant activity and phenolic content in different salvia officinalis l. extracts. Bulletin UASVM Food Science and Technology, 72(2), 2015, p. 210-214.
[19] CHERUIYOT, E., MUMERA, L., NG’ETICH, W., HASSANALI, A. and WACHIRA, F. Polyphenols as Potential Indicators for Drought Tolerance in Tea (Camellia sinensis L.). Bioscience, Biotechnology and Biochemistry, 71(9), 2007, p. 2190-2197, DOI: 10.1271/bbb.70156. En línea. http://dx.doi.org/10.1271/bbb.70156.
[20] MACHADO, M., FELIZARDO, C., FERNANDES-SILVA, A., NUNES, F. and BARROS, A. Polyphenolic compounds, antioxidant activity and L-phenylalanine ammonia-lyase activity during ripening of olive cv. “Cobrançosa” under different irrigation regimes. Food Research International, 51, 2013, p. 412–421.
[21] KOBAYASHI, T., KURATA, R. and KAI, Y. Seasonal Variation in the Yield and Polyphenol Content of Sweet Potato (Ipomoea batatas L.) Foliage. The Horticulture Journal, 88(2), 2019, p. 270–275. 2019.
[22] SILVA, D., OLIVEIRA, K., AROEIRA, L., CHAVES, D., PONCIANO, M., BRAGA, A. and LIMA, D. Chemical composition of Caatinga potential forages species. Tropical and Subtropical Agroecosystems, 18, 2015, p. 267 – 272.
[23] ANGAPPAN, R., ANANTH, D. and THILAGAR, S. Diuretic effect of chlorogenic acid from traditional medicinal plant Merremia emarginata (Burm. F.) and its by product hippuric acid. Clinical Phytoscience, 4, 2018, p. 29.
[24] REGNAULT, C., RIBODEAU, M., HAMRAOUI, A., BAREAU, I., BLANCHARD, P., GIL, M. and BARBERAN, F. Polyphenolic compounds of Mediterranean Lamiaceae and investigation of orientational effects on Acanthoscelides obtectus (Say). Journal of Stored Products Research, 40, 2004, p. 395–408.
[25] MITHÖFER, A., BOLAND, W. and MAFFEI, M. Chemical Ecology of Plant-Insect Interactions. Annual Plant Reviews Online, 34, 2018, p. 261–291. doi:10.1002/9781119312994.
[26] WAKABAYASHI, K., OGURO, M., ITAGAKI, T. and SAKAI, S. Floral-induced and constitutive defense against florivory: a comparison of chemical traits in 12 herb species. Plant Ecology, 219(8), 2018, p. 985–997.
[27] ISLAM, M., YOSHIMOTO, M., YAHARA, S., OKUNO, S., ISHIGURO, K. and YAMAKAWA, O. Identification and characterization of foliar polyphenolic composition in sweetpotato (Ipomoea batatas L.) genotypes. Journal of Agricultural and Food Chemistry, 50(13), 2002, p. 3718–3722.
Cómo citar
Salgado Chávez, J. A., Palacio Valencia, A. E., & Valero Valero, N. O. (2020). Actividad antioxidante e influencia del periodo climático sobre el contenido de polifenoles totales en Merremia aegyptia . Biotecnología En El Sector Agropecuario Y Agroindustrial, 18(2), 82–93. https://doi.org/10.18684/BSAA(18)82-93
Publicado
2020-06-16
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Artículos de Investigaciòn
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