Susceptibility in vitro to cyprofloxacin in biofilm of Pseudomonas aeruginosa and planktonic stocks. Cross-sectional study.
Abstract
Introduction: Bacterial biofilms are a serious problema in health care, mainly in infections associated with health care. They have a high capacity to adapt to the environment and nutritional requirements. In addition, due to its composition and low concentrations of antibiotics, it generates tolerance that prevents its growth from being inhibited.
Objective: To determine the in vitro susceptibility to ciprofloxacin in Pseudomonas aeruginosa biofilms. Methods: The susceptibility of the biofilm of 7 strains of Pseudomonas aeruginosa belonging to the strain collection of the University of Boyacá obtained from clinical samples of urinary origin will be prolonged using the microtiter plate method.
Results: An increase in tolerance was demonstrated in 6 strains of the 7 analyzed, reaching a decrease in growth up to a concentration of 4 μg/ml compared to its planktonic form, which modified inhibit growth up to a concentration of 14 μg/ml. As for the single resistant strain in its planktonic form, no significant changes were observed.
Conclusions: One of the analyzed biofilms presented a different behavior to ciprofloxacin, probably due to its genotype, microenvironment and/or its growth form.
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Infectious diseasesReferences
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