Phenotypic and molecular characterization of fusarium spp. coming from sesame production zone in Venezuela

  • Hernán Laurentin Universidad Centroccidental Lisandro Alvarado.
  • Gilberto Alvarado Universidad Centroccidental Lisandro Alvarado,
  • Pastora Querales Universidad Centroccidental Lisandro Alvarado.
  • Dilcia Ulacio Universidad Centroccidental Lisandro Alvarado.
  • Ana Gómez Universidad Centroccidental Lisandro Alvarado.
  • Naileth Méndez Universidad Centroccidental Lisandro Alvarado.
Palabras clave: Fusarium wilt, F. solani, F. oxysporum, F. semitectum, RAPD


Sesame is an important crop in tropical areas, which is seriously affected by soil-borne fungi such as Macrophomina phaseolina and Fusarium sp. In order to identify and characterize Fusarium spp. causing disease in sesame plants in the field, ten isolates were obtained. Eleven distinctive taxonomic traits were determined, and additionally growth rate was measured in Petri dishes. Random Amplified Polymorphic DNA (RAPD) by means of five primers was also performed. Three Fusarium species were reported: Fusarium oxysporum (2 isolates), Fusarium solani (7 isolates) and Fusarium semitectum (1 isolate). Previous reports indicate that sesame is affected just by F. oxysporum f. sp. sesami, only one previous report indicate that also F. solani causes fusarium wilt in this crop. In vitro growth rates were within a range of 0,21-0,29 mm h-1, with no trend according to the species. Growth of one of the isolates of F. oxysporum was statistically (P<0.05) faster than the others. Fifty RAPD bands (92% polymorphic) were not able to differentiate the three Fusarium species. Similarity Jaccard coefficient ranged between 0,15 and 0,47 with an average of 0,32.


Los datos de descargas todavía no están disponibles.



Biografía del autor/a

Gilberto Alvarado, Universidad Centroccidental Lisandro Alvarado,
Decanato de Agronomía, Departamento de Ciencias Biológicas. Ingeniero Agrónomo.
Pastora Querales, Universidad Centroccidental Lisandro Alvarado.
Decanato de Agronomía, Departamento de Ciencias Biológicas. Doctora en Ciencias.
Dilcia Ulacio, Universidad Centroccidental Lisandro Alvarado.
, Decanato de Agronomía, Departamento de Ciencias Biológicas. Doctora en Fitopatología (†).
Ana Gómez, Universidad Centroccidental Lisandro Alvarado.
Decanato de Agronomía, Departamento de Ciencias Biológicas. Técnico Superior Universitario Agroindustrial.
Naileth Méndez, Universidad Centroccidental Lisandro Alvarado.
Decanato de Agronomía, Departamento de Ciencias Biológicas. Técnico Superior Universitario Agroindustrial.

Referencias bibliográficas

AHIRWAR, K., PANDA, S. and JYOTISHI, A. Optimisation of sesame (Sesamum indicum L.) production through integrated nutrient management. International Journal of Current Microbiology and Applied Science, 6, 2017, p. 1701-1707.

THAKU, V., PAROHA, S. and MISHRA, R. Chemical characterization and fatty acid composition of different sesame varities. International Journal og Current Microbiology and Applied Science, 6, 2017, p. 1936-1943.

BODOIRA, R., VELEZ, A., ANDREATTA, A., MARTINEZ, M. and MAESTRI, D. Extraction of bioactive compounds from sesame (Sesamum indicum L.) defatted seeds using water and ethanol under sub-critical conditions. Food Chemistry, 237, 2017, p. 114-120.

PANDAY, A., RAJANI, B., SURABHI, J. and RANGANATHA, A. Efficacy and economics of different insecticidal treatments for the management of major sucking insect pests of sesame. Journal of Entomology and Zoology Studies, 6, 2018, p. 1247-1252.

KHAMARI, B., BEURA, S., SUSHREE, A. and MONALISA, S. Assessment of combined effect of Macrophomina phaseolina and Fusarium oxysporum on disease incidence of sesame (Sesamum indicum L.). International Journal og Current Microbiology and Applied Science, 2017, p. 1135-1139.

ORTONEDA, M., GUARRO, J., MADRID, M. CARACUEL, Z., RONCERO, M.I., MAYAYO, E. and DI PRIETO, A. Fusarium oxysporum as a multihost model for the genetic dissection of fungal virulence in plants and mammals. Infection and Immunity, 72, 2004, p. 1760-1766.

RANA, A., SAHGAL, M. and JOHRI, B. Fusarium oxysporum: genomics, diversity and plant-host interaction. In: Satyanarayana T., Deshmukh S., Johri B. (eds) Developments in Fungal Biology and Applied Mycology. Singapore (Singapore): Springer, 2017, p. 159-199.

PINEDA, J. II Curso sobre producción de ajonjolí, soya y otras leguminosas. Caracas (Venezuela): Instituto Nacional de Investigaciones Agrícolas – Universidad Centroccidental Lisandro Alvarado, 2002, 179 pp.

NELSON, P.E., DIGNAN, M.C. and ANAISSIE, E.J. Taxonomy, Biology and Clinical Aspects of Fusarium species. Clinical Microbiology Reviews, 7, 1994, p. 479.

WINDELS, C.E. Fusarium. In: Singleton L, Mihail J, Rush C (eds.) Methods for Research on Soilborne Phytopathogenic Fungi. Minnesota (USA): American Phytopathological Society Press, St. Paul, 1992, p. 115-128.

FIGUEIREDO, B. Estudos sobre a aplicação do método de Castellani para conservação de fungos patógenos em plantas. O Biológico, São Paulo, 33, 1967, p. 9-12.

NELSON, P.E., TOUSSOUN, T.A. and MARASAS, W. Fusarium species. An illustrated manual for identification. Pennsylvania (USA): Pennsylvania State University Press, 1983, 145 p.

LESLIE, J.F. and SUMMERELL, B.A. The Fusarium Laboratory Manual. Sidney (Australia): Blackwell Publisher, 2006, 120 p.

HOISINGTON, D., KHARIALLAH, M. and GONZALES, D. Laboratory Protocols: CIMMYT Applied Molecular Genetics Laboratory. 2 ed. Mexico D.F (México): 1994, 73 p.

ZAMANI, M., MOTALLERI, N. and ROSTAMIAN, A. Characterization of iranian isolates of Fusarium oxysporum on the basis of RAPD analysis, virulence and vegetative compatibility. Journal of Phytophatothology, 152, 2004, p. 449-453.

EL-BRAMAWY, M. Inheritance of resistance to Fusarium wilt in some sesame crosses under field conditions. Plant Protection Sciences, 42, 2006, p. 99-105.

SILME, R. and CAGIRGAN, M. Screening for resistance to Fusarium wilt in induced mutants and world collection of sesame under intensive management. Turkish Journal of Field Crops, 15, 2010, p. 89-93.

JYOTHI, B., ANSARI, N., VIJAY, Y., ANURADHA, G., SARKAR, A., SUDHAKAR, R. and SIDDIQ, E. Assessment of resistance to Fusarium wilt disease in sesame (Sesamum indicum L.) germplasm. Australasian Plant Pathology, 40, 2011, p. 471-475.

FALLAHPORI, A., AMINIAN, H. and ESMAILZADEH, S. Evaluation of peroxidase activity in two resistant and susceptible sesame germplasms to Fusarium damping-off caused by Fusarium oxysporum f.sp. sesami. Iran Journal of Plant Pathology, 49, 2013, p. 35-36.

LI, D., WANG, L., ZHANG, Y., LV, H., QI, X., WEI, W. and ZHANG, X. Pathogenic variation and molecular characterization of Fusarium species isolated from wilted sesame in China. African Journal of Microbiology Research, 6, 2012, p. 149-154.

GAGOSH, B., WOODWARD, S, MUR, L., AKRAM, A., ARSHAD, M., SAQULAN, S. and AKHUND, S. Identification and pathogenicity of Fusarium species associated with sesame (Sesamum indicum L.). Physiological and Molecular Plant Pathology, 102, 2018, p. 128-135.

AOKI, T., O´DONNELL, K. and GEISER, D. Systematics of key phytopathogenic Fusarium species: current status and future challenges. Journal of General Plant Pathology, 80, 2014, p. 189-201.

EL-ZAHER, E., MAHMOUD, Y. and ALY, M. Effect of different concentrations of phenol on growth of some fungi isolated from contaminated soils. African Journal of Biotechnology, 10, 2011, p. 1384-1392.

TAO, X., SHANG, B., DONG, H., CHEN, Y. and XIN, H. Effect of digestate from swine manure digester on in vitro growth of crop fungal pathogens: a laboratory study. Transactions of the ASABE, 57, 2014, p. 1803-1810.

REHMAN, A., REHMAN, A., JAVED, N., ULLAH, A. and MEHBOOB, S. Toxin production by Fusarium solani from declining citrus plants and its management. African Journal of Biotechnology, 119, 2012, p. 2199-2203.

LI, Y. Biology characteristic determination of Fusarium semitectum in soybeans. Advances in Biomedical Engineering, 3-5, 2006, p. 76-80.

MARTÍNEZ-HILDERS, A. y LAURENTIN, H. Caracterización fenotípica y molecular de Macrophomina phaseolina (Tassi) Goid. proveniente de la zona de producción de ajonjolí en Venezuela. Bioagro, 24, 2012, p. 187-196.

LUNA, P.A., SILVA, R., MARBÁN, M. y VALADEZ, M. Variabilidad genética de Fusarium oxysporum Schlechtend:FR. f. sp. ciceris (Padwick) Matuo y К. Sato mediante PCR-RAPD's en el Bajío México. Revista Mexicana de Fitopatología, 22, 2004, p. 44-51.

VAKALOUNAKIS, D. and FRAGKIADAKIS, G. Genetic diversity of Fusarium oxysporum isolates from cucumber: differentiation by pathogenicity, vegetative compatibility, and RAPD fingerprinting. Phytopathology, 89, 1999, p. 161-168.

DATTA, J. and LAL, N. Application of molecular markers for genetic discrimination of Fusarium wilt pathogen races affecting chickpea and pigeonpea in major regions of India, Cellular and Molecular Biology, 58, 2012, p. 55-65.

Cómo citar
Laurentin, H., Alvarado, G., Querales, P., Ulacio, D., Gómez, A., & Méndez, N. (2019). Phenotypic and molecular characterization of fusarium spp. coming from sesame production zone in Venezuela. Biotecnología En El Sector Agropecuario Y Agroindustrial, 17(1), 74–81.
Artículos de Investigaciòn
QR Code