Phenotypic and molecular characterization of fusarium spp. coming from sesame production zone in Venezuela

  • Hernán Laurentin Universidad Centroccidental Lisandro Alvarado.
  • Gilberto Alvarado Universidad Centroccidental Lisandro Alvarado,
  • Pastora Querales Universidad Centroccidental Lisandro Alvarado.
  • Dilcia Ulacio Universidad Centroccidental Lisandro Alvarado.
  • Ana Gómez Universidad Centroccidental Lisandro Alvarado.
  • Naileth Méndez Universidad Centroccidental Lisandro Alvarado.
Palabras clave: Fusarium wilt, F. solani, F. oxysporum, F. semitectum, RAPD

Resumen

Sesame is an important crop in tropical areas, which is seriously affected by soil-borne fungi such as Macrophomina phaseolina and Fusarium sp. In order to identify and characterize Fusarium spp. causing disease in sesame plants in the field, ten isolates were obtained. Eleven distinctive taxonomic traits were determined, and additionally growth rate was measured in Petri dishes. Random Amplified Polymorphic DNA (RAPD) by means of five primers was also performed. Three Fusarium species were reported: Fusarium oxysporum (2 isolates), Fusarium solani (7 isolates) and Fusarium semitectum (1 isolate). Previous reports indicate that sesame is affected just by F. oxysporum f. sp. sesami, only one previous report indicate that also F. solani causes fusarium wilt in this crop. In vitro growth rates were within a range of 0,21-0,29 mm h-1, with no trend according to the species. Growth of one of the isolates of F. oxysporum was statistically (P<0.05) faster than the others. Fifty RAPD bands (92% polymorphic) were not able to differentiate the three Fusarium species. Similarity Jaccard coefficient ranged between 0,15 and 0,47 with an average of 0,32.

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Biografía del autor/a

Gilberto Alvarado, Universidad Centroccidental Lisandro Alvarado,
Decanato de Agronomía, Departamento de Ciencias Biológicas. Ingeniero Agrónomo.
Pastora Querales, Universidad Centroccidental Lisandro Alvarado.
Decanato de Agronomía, Departamento de Ciencias Biológicas. Doctora en Ciencias.
Dilcia Ulacio, Universidad Centroccidental Lisandro Alvarado.
, Decanato de Agronomía, Departamento de Ciencias Biológicas. Doctora en Fitopatología (†).
Ana Gómez, Universidad Centroccidental Lisandro Alvarado.
Decanato de Agronomía, Departamento de Ciencias Biológicas. Técnico Superior Universitario Agroindustrial.
Naileth Méndez, Universidad Centroccidental Lisandro Alvarado.
Decanato de Agronomía, Departamento de Ciencias Biológicas. Técnico Superior Universitario Agroindustrial.

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Cómo citar
Laurentin, H., Alvarado, G., Querales, P., Ulacio, D., Gómez, A., & Méndez, N. (2019). Phenotypic and molecular characterization of fusarium spp. coming from sesame production zone in Venezuela. Biotecnología En El Sector Agropecuario Y Agroindustrial, 17(1), 74-81. Recuperado a partir de https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/1207
Publicado
2019-01-01
Sección
Artículos de Investigaciòn