Producción de embriones transgénicos bovinos por microinyección de un vector lentiviral pre y pos fertilización

  • Rafael Otero Arroyo Universidad de Sucre
  • Darwin Hernández Herrera Universidad de Sucre
  • Jair Pérez Universidad de La Salle
Palabras clave: Modificación genética, Proteína verde fluorescente, Transgénesis.

Resumen

El objetivo de esta investigación fue comparar la eficiencia de la microinyección de un vector lentiviral portador del gen eGFP en ovocitos maduros (PRE) y en cigotos (POS) seis horas después de la fertilización in vitro (FIV). Para ello, 1777 ovocitos maduros, fueron distribuidos en 7 tratamientos, uno control (T1), tres PRE (T2PRE, T3PRE y T4PRE) y tres POS (T2POS, T3POS y T4POS) los cuales fueron microinyectados antes (PRE) y después (POS) de la FIV. Los tratamientos T4PRE y T4POS fueron microinyectados con el vector lentiviral. Se evaluó la tasa de producción de blastocistos al día ocho y la expresión de la proteína eGFP en T4PRE y T4POS. No se encontraron diferencias en la tasa de producción de blastocistos entre el tratamiento T1 (20,2%), los controles de cultivo (T2PRE y T2POS) y los controles de microinyección (T3PRE y T3POS). La presencia del vector lentiviral disminuyó (p<0,05) la tasa de formación de blastocistos en los grupos T4PRE y T4POS (5,5 y 3,5%, respectivamente). Todos los blastocistos producidos en T4PRE y el 71,4% producidos en el T4POS expresaron la proteína eGFP (p<0,05), principalmente en el trofoectoderma y en la masa celular interna del blastocisto.

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Biografía del autor/a

Rafael Otero Arroyo, Universidad de Sucre

Facultad de Ciencias Agropecuarias, Grupo de Investigación en Reproducción y Mejoramiento Genético Animal. Ph.D. Ciencias Veterinarias.

Darwin Hernández Herrera, Universidad de Sucre

Facultad de Ciencias Agropecuarias, Grupo de Investigación en Reproducción y Mejoramiento Genético Animal. Ph.D. Ciencias Veterinarias.

Jair Pérez, Universidad de La Salle

Facultad de Ciencias Agropecuarias. Ph.D. Ciencias Agropecuarias; Ph.D. Reproducción animal.

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Cómo citar
Otero Arroyo, R., Hernández Herrera, D., & Pérez, J. (2019). Producción de embriones transgénicos bovinos por microinyección de un vector lentiviral pre y pos fertilización. Biotecnología En El Sector Agropecuario Y Agroindustrial, 18(1). Recuperado a partir de https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/1412
Publicado
2019-12-31
Sección
Artículos de Investigaciòn