Clonación del cDNA codificante de las subunidades de la gonadotropina luteinizante de Pimelodus grosskopfii

  • Andres Montoya López Asociación Colombiana de Acuicultores https://orcid.org/0000-0002-9607-6450
  • James Betancur López Universidad Nacional de Colombia
  • María Fernanda Flórez Asociación Colombiana de Acuicultores (ASOACUICOLA) https://orcid.org/0000-0001-6945-5212
  • Ana María Pérez O´brien Universidad Nacional de Colombia
  • Neil Vásquez Araque Asociación Colombiana de Acuicultores (ASOACUICOLA)
Palabras clave: Glicoproteínas, Secuencia codificante, Siluriformes

Resumen

Pimelodus grosskopfii, es un Siluriforme importante en la pesca continental en Colombia y de interés en la acuicultura. La presente investigación tuvo como objetivo clonar y determinar la secuencia codificante de las subunidades GP-α y LHβ de la gonadotropina luteinizante de esta especie. Para lograr lo anterior, el ARN total de glándulas pituitarias fue extraído y se realizó síntesis de cDNA. Los productos de PCR purificados de GP-α y LHβ fueron ligados en pJET1.2/blunt® seguido de la transformación de Escherichia coli DH5α® y posterior secuenciación de fragmentos de interés. El marco abierto de lectura de las subunidades GP-α y LHβ fue de 351 pb (116 aminoácidos) y 423 pb (140 aminoácidos), respectivamente. Se encontró una alta homología (aminoácidos) en la subunidad GP-α con otras familias de Siluriformes (87-99%) y menor homología con órdenes como Perciformes, Salmoniformes y Cypriniformes (66-90%). De igual manera para la subunidad LHβ, la homología (aminoácidos) fue alta al interior del orden Siluriformes (91-97%) y menor con otros ordenes (65-86%). Los resultados obtenidos en el presente estudio ofrecen información básica sobre las secuencias de las gonadotropinas en bagres neotropicales y pueden ser utilizados para el desarrollo de hormonas recombinantes para optimizar el control reproductivo en P. grosskopfii.

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Citas

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Cómo citar
Montoya López, A., Betancur López , J., Flórez , M. F., Pérez O´brienA. M., & Vásquez Araque, N. (2020). Clonación del cDNA codificante de las subunidades de la gonadotropina luteinizante de Pimelodus grosskopfii. Biotecnología En El Sector Agropecuario Y Agroindustrial, 18(2), 37-47. https://doi.org/10.18684/BSAA(18)37-47
Publicado
2020-06-16
Sección
Artículos de Investigaciòn