Lipase of Pachira speciosa seeds immobilized in chitosan beads: a recyclable bio-catalytic system

  • Dary Mendoza Meza Universidad del Atlántico
  • Ivon Esher Valenzuela Jaramillo Universidad del Atlántico
Palabras clave: Lipasa vegetal, Inmovilización, Actividad Lipolitica, Biocatalizador, Quitosano, Pachira speciosa, Bioconversion, Actividad residual, Esferas magnéticas, Alginato

Resumen

Las lipasas vegetales son biocatalizadores altamente versátiles debido a su quimioselectividad, enantioselectividad y regioselectividad. El propósito fue obtener un sistema biocatalítico reciclable a partir de lipasas de semillas de Pachira speciosa, aplicable a la biotransformación de lípidos. Se obtuvo una lipasa parcialmente purificada de extractos de semillas de P. speciosa, mediante cromatografía de filtración en gel en Sephadex G-100; la actividad específica lipasa (ALe) se determinó por el método de titulación de ácidos grasos libres. Se aplicó un diseño de superficie de repuesta Box-Behnken para establecer las condiciones que maximizan la inmovilización de la lipasa a tres soportes: esferas de quitosano (Q), esferas de alginato de calcio cubiertas con quitosano (Alg-Q) y esferas magnéticas de quitosano-Fe(OH)3 (Q-Fe). La mayor ALe de la enzima libre fue 0,49±0,01U/mg, a 40 °C y pH 9. El porcentaje de inmovilización y la ALe de cada biocatalízador fue: Q = 90,6 % y 3,74±0,3 nKat/mg; Alg-Q = 88,5 % y 3,62±0,1 nKat/mg; EQ-Fe = 76,4 % y 2,88±0,1 nKat/mg. El sistema biocatalítico más estable fue la lipasa inmovilizada en quitosano, con 85 % de rentención de la ALe hasta el tercer ciclo catalítico. Estudios futuros estarán enfocados a establecer los parámetros cinéticos del nuevo biocatalizador.

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Cómo citar
Mendoza Meza, D., & Valenzuela Jaramillo, I. E. (2021). Lipase of Pachira speciosa seeds immobilized in chitosan beads: a recyclable bio-catalytic system. Biotecnología En El Sector Agropecuario Y Agroindustrial, 1-12. https://doi.org/10.18684/rbsaa.v.n.2022.1890
Publicado
2021-10-21
Sección
Artículos de Investigaciòn

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