Producción biocatalítica de alcohol perílico utilizando células completas de aspergillus niger dsm 821

  • Claudia Cristina Ortiz
  • Yaneth Perea Universidad Industrial de Santander.
  • Claudia Cristina Hurtado Universidad Industrial de Santander.
Palabras clave: Biotransformación, Bioconversión, (R)-( )-limoneno, Aspergillus niger.

Resumen

En esta investigación se estudió la producción de alcohol pen'lico (AP) mediante

biotransformación del (R)-(+)-limoneno utilizando células completas de Aspergillus niger DSM 821 a escala de laboratorio. Para tal fin se determinó la influencia

de diferentes variables sobre la selectividad del microorganismo hacia un produto determinado y el rendimiento de biotransformación. Se evaluó el efecto de la

fase del crecimiento del hongo y el efecto inductor del sustrato. De igual forma

se incluyeron las siguientes variables: pH, medio de biotransformación y con

centración del sustrato. La mayor velocidad específica de crecimiento se alcanzó

utilizando Agar Extracto Malta (MEA) como medio de cultivo a 28°C. Además se

obtuvieron 246 mg/L de AP utilizando 12 mM de (R)-(-\-)- limoneno en caldo

Malta y Extracto de Levadura (MYB). Esta producción fue 1,9 y 3,1 veces ma

yor que la obtenida en caldo de Levadura, Extracto de Malta, Peptona y Glucosa

(YMPG) y Caldo de Extracto de Levadura y Glucosa (YG), respectivamente. Las

mayores concentraciones de AP (405 mg/L) fueron obtenidas cuando A. niger

DMS 821 fue cultivado en medio suplementado con 50 mL de limoneno durante 

la fase exponencial (a las 12 h), en MYB a pH 5,0, 28°C, 300 rpm y 6 días

de biotransformación. Adicionalmente se obtuvieron otros subproductos:

limoneno-1,2-diol, linalol, carvona, fenil etanol y etil esteres de ácidos pal-

mitico, oleico y linoleico.

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Lenguajes:

es

Biografía del autor/a

Yaneth Perea, Universidad Industrial de Santander.
Escuela de Química. Química PhD.
Claudia Cristina Hurtado, Universidad Industrial de Santander.

Escuela de Bacteriología y Laboratorio Clínico. Microbióloga
PhD.

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Cómo citar
Ortiz, C. C., Perea, Y., & Hurtado, C. C. (2014). Producción biocatalítica de alcohol perílico utilizando células completas de aspergillus niger dsm 821. Biotecnología En El Sector Agropecuario Y Agroindustrial, 12(1), 36–46. Recuperado a partir de https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/312
Publicado
2014-07-01
Sección
Artículos de Investigaciòn
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