Enzimatic hydrolysis of Bactris guineensis oil with Carica papaya latex fractions: study of the effect of temperature, ph and substrate concentration
Keywords:
Fatty acid, Lipolytic activity, Bioconversion, Vegetable lipases
Abstract
Carica papaya lipases are biocatalysts with potential for lipid bioconversion. This research studied the lipolitic activity (LA) of the crude powder (P3) and protease-free (CPL-p) fractions of C. papaya latex on Bactris guineensis (corozo) oil. Latex and oil were obtained from agro-industrial waste. A composite central design allowed to establish the temperature (T) and pH effect on LA of P3 and CPL-p; thermal, pH stabilities and kinetic parameters were also determined. The maximum LA were 722,43 ± 1,92 U/g (T= 40°C y pH= 9) and 518,65 ± 4,70 U/g (T = 54°C y pH= 8) for P3 and CPL-p respectively. P3 showed higher affinity (KM = 1,3x104 ± 1,3x103 µM y Vmax = 18,66 ± 0,47 µmol/min) compared to CPL-p (KM = 2,6x104 ± 1,1x103 µM y Vmax = 16,58 ± 0,08 µmol/min); similarly, greater efficiency in hydrolysis, due to the high content of medium chain fatty acids (lauric and miristic acid > 80%). As a conclusion, the fraction P3 is an efficient biocatalyst for the bioconversion of corozo oil, demonstraring that the residues of these fruits are valuable resources for its production chains.
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Disciplines:
Biotechnology, Enzymology, Enzymatic catalysis, agroindustryReferences
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[22] DAYRIT, F.M. Lauric acid is a medium-chain fatty acid; coconut oil is a medium-chain triglyceride. Philippine journal of science, 143(2), 2014, p. 157-166.
[23] Pham, L.J. et al. Triacylglycerol and regiospecific fatty acid analyses of Philippine seed oils. JAOCS, 75(7), 1998, p. 807-811, DOI: 10.1007/s11746-998-0230-5.
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[26] NIELSEN, J.E. and MCCAMMON, J.A. Calculating pka values in enzyme active sites. Protein science, 12(9), 2003, p. 1894-1901, DOI: 10.1110/ps.03114903.
[27] DANIEL, R.M. and DANSON, M.J. Temperature and catalytic activity of enzymes: A fresh understanding. FEBS Letters, 587(17), 2013, p. 2738-2743, DOI: 10.1016/j.febslet.2013.06.027.
[2] DAYRIT, F.M. The properties of lauric acid and their significance in coconut oil. JAOCS, 92(1), 2015, p. 1-15, DOI: 10.1007/s11746-014-2562-7.
[3] MCCARTY, M.F. and DINICOLANTONIO, J.J. Lauric acid rich medium chain triglycerides can substitute for other oils in cooking applications and may have limited pathogenicity. Open heart, 3(2), 2016, p. 1–5, DOI: 10.1136/openhrt-2016-000467.
[4] KABARA, J.J. et al. Fatty acids and derivatives as antimicrobial agents. Antimicrobial agents and chemotherapy, 2(1), 1972, p. 23-28, DOI: 10.1128/aac.2.1.23.
[5] SARUP, R., SINGH, T. and PANDEY, A. En: Advances in enzyme technology. Microbial enzymes-an overview. 1 ed. Reino Unido: Elsevier, 2019, p. 1-40.
[6] ABDELKAFI, S. et al. Carica papaya lipase: A naturally immobilized enzyme with interesting biochemical properties. Plant foods for human nutrition, 66(1), 2011, p. 34-40, DOI:10.1007/s11130-010-0206-0.
[7] RIVERA, I. y SANDOVAL, G. Caracterización de diversas fracciones del látex Carica papaya como biocatalizadores en la hidrólisis de triglicéridos. Grasas y aceites, 65(1), 2014, p. 1-8, DOI: 10.3989/gya.049313.
[8] RIVERA, I., MATEOS, J.C. and SANDOVAL, G. En: Lipases and phospholipases. Methods in molecular biology (methods and protocols) plant lipases: partial purification of Carica papaya lipase. 1 ed. New York (USA): Human press, 2012, p. 115-122.
[9] ABDELKAFI, S. et al. Identification and biochemical characterization of a GDSL-motif carboxylester hydrolase from Carica papaya latex. Biochimica et biophysica acta (BBA)-molecular and cell biology of lipids, 1791(11), 2009, p. 1048–1056, DOI: 10.1016/j.bbalip.2009.06.002.
[10] ABDELKAFI, S. et al. Identification of a new phospholipase D in Carica papaya latex. Gene, 499(2), 2012, p. 243-249, DOI: 10.1016/j.gene.2012.03.033.
[11] RIVERA, I. et al. Functional expression, extracellular production, purification, structure modeling and biochemical characterization of Carica papaya lipase 1. Process biochemistry, 56, 2017, p. 109-116, DOI: 10.1016/j.procbio.2017.02.009.
[12] PECHA, J. et al. A kinetics study of the simultaneous methanolysis and hydrolysis of triglycerides. Chemical engineering journal, 288, 2016, p. 680-688, DOI: 10.1016/j.cej.2015.12.033.
[13] COLOMBIA. MINISTERIO DE AGRICULTURA Y DESARROLLO RURAL. Cadena productiva papaya-área, producción y rendimiento. Bogotá (Colombia): 2019.
[14] Hernández, C. et al. Efecto de la refinación física sobre el aceite de la almendra del corozo (Acrocomia aculeata). Información tecnológica, 18(4), 2007, p. 59-68, DOI: 10.4067/S0718-07642007000500008.
[15] ESPAÑA. INTERNATIONAL ORGANIZATION OF STANDARDIZATION. Animal and vegetable fats and oils-preparation of methyl esters of fatty acids. Madrid (España): 2000.
[16] ESPAÑA. INTERNATIONAL ORGANIZATION OF STANDARDIZATION. Animal and vegetable fats and oils-analysis by gas chromatography of methyl esters of fatty acids. Madrid (España): 1990.
[17] ORTEGA, V., FONTALVO, M. y MENDOZA, D. Comparación de diferentes fracciones del látex de Carica papaya L., y Novozyme 435 en la metanólisis del aceite de canola. Revista de Ciencias, 22(1), 2018, p. 75-90, DOI: 10.25100/rc.v22i1.7102.
[18] COLOMBIA. GOBERNACIÓN DEL TOLIMA. Protocolo de buenas prácticas de para poscosecha de papaya Carica papaya L. Ibagué (Colombia): 2017.
[19] SAMBROOK, J. and RUSSELL, D. SDS-Polyacrylamide gel electrophoresis of proteins. CSH Protocols, 4, 2006, DOI: 10.1101/pdb.prot4540.
[20] Plou, F.J. et al. Analysis of tween 80 as an esterase/lipase substrate for lipolytic activity assay. Biotechnology techniques, 12(3), 1998, p. 183-186, DOI: 10.1023/A:1008809105270.
[21] VILLENEUVE, P. et al. Carica papaya latex lipase: sn‐3 stereoselectivity or short‐chain selectivity? Model chiral triglycerides are removing the ambiguity. JAOCS, 72(6), 1995, p. 753-755, DOI: 10.1007/BF02635668.
[22] DAYRIT, F.M. Lauric acid is a medium-chain fatty acid; coconut oil is a medium-chain triglyceride. Philippine journal of science, 143(2), 2014, p. 157-166.
[23] Pham, L.J. et al. Triacylglycerol and regiospecific fatty acid analyses of Philippine seed oils. JAOCS, 75(7), 1998, p. 807-811, DOI: 10.1007/s11746-998-0230-5.
[24] BEZARD, J.A. The component triglycerides of palm‐kernel oil. Lipids, 6(9), 1971, p. 630-634, DOI: 10.1007/BF02531519.
[25] ROBINSON, P.K. Enzymes: Principles and biotechnological applications. Essays in biochemistry, 59, 2015, p. 1-41, DOI: 10.1042/bse0590001.
[26] NIELSEN, J.E. and MCCAMMON, J.A. Calculating pka values in enzyme active sites. Protein science, 12(9), 2003, p. 1894-1901, DOI: 10.1110/ps.03114903.
[27] DANIEL, R.M. and DANSON, M.J. Temperature and catalytic activity of enzymes: A fresh understanding. FEBS Letters, 587(17), 2013, p. 2738-2743, DOI: 10.1016/j.febslet.2013.06.027.
How to Cite
Urquijo Rodriguez, M., Fontalvo Gómez, M. ., Veloso de Paula, A., & Mendoza Meza, D. (2020). Enzimatic hydrolysis of Bactris guineensis oil with Carica papaya latex fractions: study of the effect of temperature, ph and substrate concentration. Biotechnology in the Agricultural and Agroindustrial Sector, 18(2), 70–81. https://doi.org/10.18684/BSAA(18)70-81
Published
2020-06-16
Section
Artículos de Investigaciòn
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