Aspergillus niger y Rhizopus oryzae inmovilizados para la producción de almidón modificado

  • Alvaro Esteban Aldana Porras Universidad del Tolima
  • Diego Fernando Montoya Yepes Universidad del Tolima
  • Walter Murillo Arango Universidad del Tolima
  • Jonh Jairo Méndez Arteaga Universidad del Tolima
Palabras clave: Hidrólisis, Esterificación, Hongos inmovilizados, Estropajo, Biotecnología para el aprovechamiento de subproductos, Almidón

Resumen

La modificación de polímeros naturales es llevada a cabo para ampliar la funcionalidad y usos industriales de los mismos. El uso de microorganismos como fuente enzimática para estas tecnologías proporciona protección de las biomoléculas frente a las condiciones del medio, reutilización, bajo costo, facilidad en su cultivo y aplicación. En este sentido, este estudio se enfocó en la inmovilización de Aspergillus niger y Rhizopus oryzae, como fuente primaria de enzimas requeridas para la acilación e hidrolisis de almidón. Esto mediante el uso de extracto de salvado de arroz como medio de crecimiento y el estropajo como soporte para la inmovilización. Se alcanzaron concentraciones de proteína de 30,7 ± 0,3 y 24,9 ± 0,3 (mg/cm3 de soporte) respectivamente, en el modelo de acilación, se observó una modificación química de los polímeros causada por las condiciones de reacción, obteniendo materiales con propiedades funcionales variables. En hidrólisis se logró un 40% de degradación, posibilitando la obtención de diferentes derivados de almidón. En este sentido, el desempeño en hidrólisis, la concentración proteica de los microorganismos, el estropajo como soporte y el extracto de salvado de arroz como medio inductor de enzimas, promueven este sistema como una alternativa económica para los procesos de modificación industrial.

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Cómo citar
Aldana Porras, A. E., Montoya Yepes, D. F., Murillo Arango , W., & Méndez Arteaga , J. J. (2021). Aspergillus niger y Rhizopus oryzae inmovilizados para la producción de almidón modificado. Biotecnología En El Sector Agropecuario Y Agroindustrial, 19(2), 69-81. https://doi.org/10.18684/bsaa.v19.n2.2021.1544
Publicado
2021-01-22
Sección
Artículos de Investigaciòn

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