Harina de larva de Mosca soldado negra (Hermetia Illucens) fermentada con Lactobacillus bulgaricus como pienso probiótico
Resumen
El uso exacerbado de antibióticos en ganado desarrolla patógenos súper resistentes, lo que exige la producción de piensos probióticos para el ganado y que a su vez sean sostenibles. Por ello, en el estudio el objetivo fue fermentar con el probiótico Lactobacillus Bulgaricus 5 mezclas de harina de larva de mosca soldado negra (HLMSN) y soya en diferentes proporciones a fin de determinar cuál permite mayor crecimiento de Lactobacillus Bulgaricus y representar una nueva alternativa de alimentación para una ganadería sustentable. Para lograr ello, se determinó la curva de crecimiento de Lactobacillus Bulgaricus en caldo MRS, en la fase exponencial de crecimiento este fue añadido a las 5 formulaciones de HLMSN y soya y finalmente se determinaron UFC producidas por cada formulación. Se obtuvo que la concentración de HLMSN posee un efecto estadísticamente significativo (p<0,05) sobre la concentración de Lactobacillus Bulgaricus, siendo que en el tratamiento de HLMSN pura se obtuvo el doble de UFC del probiótico que en el tratamiento de soya pura. Dichos resultados revelan que Lactobacillus Bulgaricus es capaz de metabolizar y producir mayor biomasa teniendo de sustrato HLMSN en vez de soya, de modo que esta formulación puede ser usada en estudios posteriores en pruebas antimicrobianas in vitro e in vivo.
Descargas
Asuntos:
EspañolDisciplinas:
fermentación, Microbiología, ProbióticosLenguajes:
Español,Referencias bibliográficas
AJAYI, AYODELE-OLUWASEUN; ODEYEMI, ADEBOWALE-TOBA; AKINJOGUNLA, OLAJIDE- JOSEPH; ADEYEYE, AKINWOLE-BABAFENWA; AYO-AJAYI, IBIWUMI. Review of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes within the One Health Framework. Infection Ecology & Epidemiology, v. 14, n. 1, 2024.https://doi.org/10.1080/20008686.2024.2312953
AKRAM, FATIMA; IMTIAZ, MEMOONA; HAQ, IKRAM-UL. Emergent crisis of antibiotic resistance: A silent pandemic threat to 21st century. Microbial Pathogenesis, v. 174, 2023.https://doi.org/10.1016/j.micpath.2022.105923
ANANY, E.M.; IBRAHIM, M.A.; EL-RAZEK, I.M.A.; EL-NABAWY, E.M.; AMER, A.A.; ZAINELDIN, A.I.; GEWAILY, M.S.; DAWOOD, M.A.O. Combined Effects of Yellow Mealworm (Tenebrio molitor) and Saccharomyces cerevisiae on the Growth Performance, Feed Utilization Intestinal Health, and Blood Biomarkers of Nile Tilapia (Oreochromis niloticus) Fed Fish Meal-Free Diets. Probiotics and antimicrobial proteins, 2023.https://doi.org/10.1007/s12602-023-10199-8
ANSARI, F.; BAHADORI, A.; SAMAKKHAH, S.A.; PIROUZIAN, H. R.; POURJAFAR, H. Probiotic Lactic Acid Bacteria. Handbook of Food Bioactive Ingredients, 2023, p. 1473-1503. https://doi.org/10.1007/978-3-031-28109-9_41
BAVA, ROBERTO; CASTAGNA, FABIO; LUPIA, CARMINE; POERIO, GIUSI; LIGUORI, GIOVANNA; LOMBARDI, RENATO; NATURALE, MARIA-DIANA; MERCURI, CATERINA; BULOTTA, ROSA-MARÍA; BRITTI, DOMENICO; PALMA, ERNESTO. Antimicrobial Resistance in Livestock: A Serious Threat to Public Health. Antibiotics, v. 13, n. 6, 2024, p. 551. https://doi.org/10.3390/antibiotics13060551
BHARDWAJ, S.; MEHRA, P.; DHANJAL, D. S.; SHARMA, P.; SHARMA, V.; SINGH, R.; NEPOVIMOVA, E.; CHOPRA, C.; KUČA, K. Antibiotics and Antibiotic Resistance- Flipsides of the Same Coin. Current pharmaceutical design, v. 28, n. 28, 2022, p. 2312–2329.https://doi.org/10.2174/1381612828666220608120238
CABRERA-GONZÁLEZ, M.A.; VÁSQUEZ-PÉREZ, H.V.; QUILCATE-PAIRAZAMÁN, C.; BAZÁN-ARCE, J.; CUEVA-RODRÍGUEZ, M. Assessment of antibiotic resistance in fecal samples from calves with diarrhea in the Cajamarca region, Perú. Revista mexicana de ciencias pecuarias, v. 14, n. 4, 2023, p. 782-795.
CHISORO, P.; JAJA, I.F.; ASSAN, N. Incorporation of local novel feed resources in livestock feed for sustainable food security and circular economy in Africa. Frontiers in Sustainability, v. 4, 2023. https://doi.org/10.3389/frsus.2023.1251179
CORENTIN, BITEAU; TOM, BRY-CHEVALIER; DUSTIN, CRUMMETT; REN, RYBA; MICHAEL, ST. JULES. Insect-based livestock feeds are unlikely to become economically viable in the near future. Food and Humanity, v. 3, 2024.https://doi.org/10.1016/j.foohum.2024.100383
ELAHI, U.; XU, C.C.; WANG, J.; LIN, J.; WU, S.G.; ZHANG, H.J.; QI, G.H. Insect meal as a feed ingredient for poultry. Animal bioscience, v. 35, n. 2, 2022, p. 332–346. https://doi.org/10.5713/ab.21.0435
FU, C.; CHEEMA, W.A.; MOBASHAR, M.; SHAH, A.A.; ALQAHTANI, M.M. Insects as Sustainable Feed: Enhancing Animal Nutrition and Reducing Livestock Environmental Impression. Journal of animal physiology and animal nutrition, 2024.https://doi.org/10.1111/jpn.14055
HASAN, I.; RIMOLDI, S.; SAROGLIA, G.; TEROVA, G. Sustainable Fish Feeds with Insects and Probiotics Positively Affect Freshwater and Marine Fish Gut Microbiota. Animals, v. 13, n. 10, 2023, p. 1633. https://doi.org/10.3390/ani13101633
HASSANI, S.; MOOSAVY, M.H.; GHARAJALAR, S.N.; KHATIBI, S.A.; HAJIBEMANI, A.; BARABADI, Z. High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk. Scientific reports, v. 12, n. 1, 2022, p. 3878. https://doi.org/10.1038/s41598-022-07845-6
JIAN, Z.; ZENG, L.; XU, T.; SUN, S.; YAN, S.; YANG, L.; HUANG, Y.; JIA, J.; DOU, T. Antibiotic resistance genes in bacteria: Occurrence, spread, and control. Journal of basic microbiology, v. 61, n. 12, 2021, p. 1049–1070. https://doi.org/10.1002/jobm.202100201
KUMAR, P.; ABUBAKAR, A.A.; VERMA, A.K.; UMARAW, P.; ADEWALE-AHMED, M.; MEHTA, N.; SAZILI, A. Q. New insights in improving sustainability in meat production: opportunities and challenges. Critical Reviews in Food Science and Nutrition, v. 63, n. 33, 2022, p. 11830–11858. https://doi.org/10.1080/10408398.2022.2096562
LEYING MENG, LIANGXIAO MA, JIAHUI XU, KEMING RONG, NAN PENG, SHUMIAO ZHAO. Effect of enzyme-assisted fermentation on quality, safety, and microbial community of black soldier fly larvae (Hermetia illucens L.) as a novel protein source. Food Research International, v. 174, n. 1, 2023.https://doi.org/10.1016/j.foodres.2023.113624
LU, S.; TAETHAISONG, N.; MEETHIP, W.; SURAKHUNTHOD, J.; SINPRU, B.; SROICHAK, T.; ARCHA, P.; THONGPEA, S.; PAENGKOUM, S.; PURBA, R.A.P.; PAENGKOUM, P. Nutritional Composition of Black Soldier Fly Larvae (Hermetia illucens L.) and Its Potential Uses as Alternative Protein Sources in Animal Diets: A Review. Insects, v. 13, n. 9, 2022, p. 831.https://doi.org/10.3390/insects13090831
MANNIELLO, M.D.; MORETTA, A.; SALVIA, R. Insect antimicrobial peptides: potential weapons to counteract the antibiotic resistance. Cellular and molecular life sciences, v. 78, 2021, p. 4259–4282.
https://doi.org/10.1007/s00018-021-03784-z
ISLAM, MANIRUL; CHUL-JU, YANG. Efficacy of mealworm and super mealworm larvae probiotics as an alternative to antibiotics challenged orally with Salmonella and E. coli infection in broiler chicks. Poultry Science, v. 96, n. 1, 2017, p. 27-34.https://doi.org/10.3382/ps/pew220
NEGI, S.; SHARMA, S. Ready to Eat Food: A Reason for Enhancement in Multidrug Resistance in Humans. Advanced pharmaceutical bulletin, v. 14, n. 3, 2024, p. 504–512. https://doi.org/10.34172/apb.2024.023
PENDRILL, F.; GARDNER, T.A.; MEYFROIDT, P.; PERSSON, U.M.; ADAMS, J.; AZEVEDO, T.; WEST, C. Disentangling the numbers behind agriculture-driven tropical deforestation. Science, v. 377, n. 6611, 2022.https://doi.org/10.1126/science.abm9267
PHAENGPHAIREE, P.; BOONTIAM, W.; WEALLEANS. Dietary supplementation with full-fat Hermetia illucens larvae and multi-probiotics, as a substitute for antibiotics, improves the growth performance, gut health, and antioxidative capacity of weaned pigs. BMC Veterinary Research, v. 19, n. 7, 2023.https://doi.org/10.1186/s12917-022-03550-8
RAHMAN, M.M.; BYANJU, B.; LAMSAL, B.P. Protein, lipid, and chitin fractions from insects: Method of extraction, functional properties, and potential applications. Critical Reviews in Food Science and Nutrition, v. 64, n. 18, 2023, p. 6415–6431.
https://doi.org/10.1080/10408398.2023.2168620
REÁTEGUI, J.; BARRIGA, X.; OBANDO, A.; MOSCOSO, G.; MANRIQUE, P.; SALAZAR, I. Harina de larva de Hermetia illucens (Diptera: Stratiomyidae) como ingrediente proteico de reemplazo parcial de harina de soja en la alimentación de Cavia porcellus (Cuy): efecto en el consumo, ganancia de peso y conversión alimenticia. Scientia Agropecuaria, v. 11, n. 4, 2020, p. 513-519.
REYES-ARQUINIGO, MILAGROS-MELISSA. Determinación de la resistencia de Escherichia coli y Staphylococcus aureus meticilina resistente aislado de quesos frescos de la localidad de Canta, Lima-Perú [Tesis Biología]. Lima (Perú): Universidad Ricardo Palma, Facultad de Ciencias Biológicas, 2023.
SARICA, Ş.; YAVUZ, M.; SANLI, E.; KATI, A.; ALTUNTAS, S.; ALATAS, M.; EKICI, M.; YAZAREL, S.; KESKINTAS, A. Effects of dietary fermented mealworm larvae and stocking density on performance, blood stress indicator and intestine parameters of broilers. Journal of the Hellenic Veterinary Medical Society, v. 75, n. 3, 2024, p. 7887–7902.https://doi.org/10.12681/jhvms.35709
SILVA, ANA-PAULA; LONGHI, DANIEL-ANGELO; DALCANTON, FRANCIELI; FALCÃO-DE-ARAGÃO, GLÁUCIA-MARIA. Modelling the growth of lactic acid bacteria at different temperatures. Brazilian archives of biology and technology, v. 61, 2018, e18160159.
SMETS, R.; VERBINNEN, B.; VAN-DE-VOORDE, I.; AERTS, G.; CLAES, J.; VAN-DER-BORGHT, M. Sequential extraction and characterisation of lipids, proteins, and chitin from black soldier fly (Hermetia illucens) larvae, prepupae, and pupae. Waste and Biomass Valorization, v. 11, 2020, p. 6455-6466.
SOGARI, G.; AMATO, M.; BIASATO, I.; CHIESA, S.; GASCO, L. The Potential Role of Insects as Feed: A Multi-Perspective Review. Animals, v. 9, n. 4, 2019, p. 119. https://doi.org/10.3390/ani9040119
SOGARI, G.; AMATO, M.; PALMIERI, R.; HADJ-SAADOUN, J.; FORMICI, G.; VERNEAU, F.; MANCINI, S. The future is crawling: Evaluating the potential of insects for food and feed security. Current research in food science, 6, 2023, 100504.https://doi.org/10.1016/j.crfs.2023.100504
SONG, M.; YUN, B.; MOON, J.H.; PARK, D.J.; LIM, K.; OH, S. Characterization of Selected Lactobacillus Strains for Use as Probiotics. Korean journal for food science of animal resources, v. 35, n. 4, 2015, p. 551–556. https://doi.org/10.5851/kosfa.2015.35.4.551
CAPUTO, VINCENZINA; SUN, JIAYU; STAPLES, AARON; TAYLOR, HANNAH. Market outlook for meat alternatives: Challenges, opportunities, and new developments, Trends in Food Science & Technology, v. 148, 2024.
https://doi.org/10.1016/j.tifs.2024.104474
XIONG, Y. L. Meat and meat alternatives: where is the gap in scientific knowledge and technology?. Italian Journal of Animal Science, v. 22, n. 1, 2023, p. 482–496. https://doi.org/10.1080/1828051X.2023.2211988
ZHANG, M.; GU, L.; ZHENG, P.; CHEN, Z.; DOU, X.; QIN, Q.; CAI, X. Improvement of cell counting method for Neubauer counting chamber. Journal of clinical laboratory analysis, v. 34, n. 1, 2020.https://doi.org/10.1002/jcla.23024
Derechos de autor 2026 Universidad del Cauca
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
Español
Inglés
.png)
